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Transfection Reagents

Transfection Reagents

針對最夯的基因剪輯CRISPR/Cas系統設計的細胞轉染試劑 新一代高轉染效率、低細胞毒性 神經細胞及幹細胞轉染試劑
DNA-In® CRISPR :
專為CRISPR/Cas9 系統設計的細胞轉染試劑,適用large CRISPR-associated  Cas9 表現載體或是 Cas9/RNA-guided 表現載體(如麻省理工學院張峰實驗室的pX458 vector )並具備高轉染效率及低細胞毒性優點。
適用細胞種類: 大部分轉染困難的primary cells, iPSCs 及常見的細胞株. 



DNA-In® CRISPR delivers Cas9-GFP expression vector with maximum efficiency 高轉染效率!
• Human primary fibroblasts
• Humanprimary keratinocytes
• Human skeletal muscle cells
• Primary human  endothelial cells (HuVEC)
四種細胞使用DNA-In® CRISPR reagent轉染pSpCas9(BB) -2A-GFP pasmid  (9.3kb)(source: Addgene),24h後固定細胞並以染anti-GFP與anti-Cas9抗體後觀察轉染效率

轉染效率比較:  DNA-In® CRISPR  完勝  L2K, L3K
• Human skeletal muscle cells 
• Human dermal primary fibroblasts
• Human primary keratinocytes
Cells were plated  to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In® CRISPR transfected cells (left image) show significantly higher Cas9  transfection efficiency vs cells transfected with competitor reagents (L2K, L3K). 
新一代高轉染效率、低細胞毒性 神經細胞及幹細胞轉染試劑

DNA-In™ Neuro
DNA-In™ Neuro 為新一代神經細胞轉染試劑,由早期產品Lipofectamine 共同發明者所開發。DNA-In™ Neuro 對神經細胞具超高DNA轉染效率及極低細胞毒性,更能增加轉染後神經元細胞的存活與神經生長。

 

•神經細胞轉染效率達 2 0% 以上
•細胞毒性超低,轉染後神經元細胞存活率大幅提升
•具穩定的效率以及可再現性

DNA-In™ Stem 
DNA-In™ Stem 專為胚胎幹細胞和成體幹細胞的 DNA 轉染而設計。包括iPSC-derived neural stem cells (NSC), Human ESC, adipose-derived mesenchymal stem cells (MSC) and Huntington's Disease (HD)-specific iPSCs


High efficiency CRISPR-Cas9 Delivery
Neural progenitor cells (HT223-B) from adult brain were transfected with pEF1a-Cas9-2A-GFP plasmid (Addgene) using DNA-In® Stem (left) and Lipofectamine 3000


mRNA-In™ Neuro
僅需少量 mRNA 即可達到相當高的轉染效率,毒性減至最低,提升細胞的存活率。
針對難以轉染的neuron及多種 primary 細胞, 提高蛋白表現量。
應用範圍廣,包括神經毒性研究 與類神經發育 (neurite development)。

 
mRNA-In™ Stem 
僅需少量 mRNA 即可達到相當高的轉染效率。
高表現、低毒性 - 在ESC&iPSC獲得高效的mRNA與蛋白表現,同時轉染後保持最佳的細胞健康度。
對多種幹細胞具高轉染效率– 如: Human ES,  iPSC-derived stem cells , Human Dermal Fibroblasts and MSC。


 
各種不同細胞專用的轉染試劑:
Cell / Application Specific
Transfection Reagents
Product Description & Application Cat. No.
DNA-In® CRISPR  Specifically designed to deliver Cas9 expression vectors or all-in-one Cas9/sgRNA expression vectors at high efficiency. GST-211
DNA-In® SY5Y  Optimized in SH-SY5Y neuroblastoma cells for maximum
transfection efficiency and viability.
GST-2151
DNA-In® HepG2  Formulated for HepG2 (human hepatocellular carcinoma)
cells.
 73761  
DNA-In® A549  Formulated specifically for A549 (adenocarcinomic human
alveolar basal epithelial) cells.
73771   
DNA-In® CHO  Optimized for adherent CHO (Chinese Hamster Ovary) cells.  73781 
DNA-In® 293   Designed for HEK 293 (Human Embryonic Kidney) cells. 73791   

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