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Diagnostics 系列原料

Dojindo 提供開發檢測平台與診斷檢驗的最佳原料選擇

生物樣品中目標元素的定量測定

1. Enzymatic Detection

(1) New Trinder’s Reagents (Oxidase system)

New Trinder 的試劑是高水溶性苯胺衍生物 (aniline derivatives)，適用於過氧化氫 (hydrogen peroxide)的比色測定。在 4-aminoantipyrine(4-AA) 等偶聯劑 (coupler)存在的情況下，Trinder 試劑會被過氧化物酶 (peroxidase)和兩分子過氧化氫 (hydrogen peroxide)氧化，並呈現紫色或藍色。

(2) Tetrazolium Salts and Electron Mediators (Dehydrogenase system)

四唑鹽 (Tetrazolium Salts)已被開發用於測定脫氫酶活性 (dehydrogenase activity)，脫氫酶氧化其底物 (substrate)並還原其輔酶NAD或NADP並產生NADH或NADPH。有色染料是在電子存在下還原 NADH 產生的。為了在水相條件下進行均質測定，水溶性四唑鹽（WST）由於比不溶於水的四唑鹽（如NBT和INT）有更好的水溶性，被廣泛應用於診斷分析和生命科學研究。

Detection Principle

ItemsPrinciple (Oxidase System) New Trinder’s ReagentsPrinciple (Dehydrogenase System) Tetrazolium Salts and Electron Mediators
Cholesterol


Creatine
Glucose

Lactic acid


NH3-

Pyruvate

Triglycerides

Uric acid

-

2. Chelate Complexes Detection

(1) Other Chromogens for Clinical Chemical Assays

Dojindo 提供多種試劑，可與生物樣本中的目標物質形成螯合複合物。這些試劑適用於樣本中目標物質的定量測定。

ItemsReagentPrinciple
CalciumChlorophosphonazo-IIICa2+ + Chlorophosphonazo-III → Ca2+ - Chlorophosphonazo-III complex
Copper3,5-DiBr-PAESACeluroplasmin – Cu2+ → Denaturation → Cu2+ → Reduction → Cu+ + 3,5-DiBr-PAESA → Cu+ - 3,5-DiBr-PAESA complex
IronNitroso-PSAPTransferrin – Fe3+ → Denaturation → Fe3+ → Reduction → Fe2+ + Nitroso-PSAP → Fe2+ - Nitroso-PSAP complex
MagnesiumXB-I (Xylidyl blue-I)Mg2+ + Xylidyl blue-I → Mg2+ - Xylidyl blue-I complex
Urinary Total ProteinPR (Pyrogallol Red)Pyrogallol Red + Molybdate(VI) + Protein → PR – MO - Protein complex
Zinc5-Br-PAPSPhysiological Ligand – Zn2+ → Denaturation → Zn2+ + 5-Br-PAPS → Zn2+ - 5-Br-PAPS complex
Nitro-PAPSZn2+ + Nitro-PAPS → Zn2+ – Nitro-PAPS complex

Oxidative Dyes (New Trinder’s Reagents )

Code#DescriptionCAS#
OC01ADOS82692-96-4
OC02ADPS82611-88-9
OC04ALPS82611-85-6
OC06DAOS83777-30-4
OC08HDAOS82692-88-4
OC11MAOS82692-97-5
OC13TOOS82692-93-1
OC14TOPS40567-80-4
OC21MADB209518-16-1
OC22TODB1044537-70-3
OC46EMSE-

Reductive Dyes (Tetrazolium Salts )

Code#DescriptionCAS#
W201WST-1150849-52-8
W202WST-3515111-36-1
W203WST-4178925-54-7
W204WST-5178925-55-8
W217WST-9-
I003INT146-68-9
M009MTT298-93-1
N011Nitro-TB298-83-9
T012TB1871-22-3

Oxidative Dyes (Others)

Code#DescriptionCAS#
K257KN-304-
D006DAB7411-49-6
S302SAT-3679787-07-6
T022TMBZ54827-17-7
T036TMBZ-PS102062-46-4, 102062-36-2(free acid)

Electron Mediators

Code#DescriptionCAS#
M0031-Methoxy PMS65162-13-2
M4701-Methoxy PES133395-54-7

Chromogens for Chelate Complexes Detection

Code#DescriptionCAS#
C010Chlorophosphonazo-III1914-99-4
D0413,5-DiBr-PAESA100743-65-5
N010Nitroso-PSAP80459-15-0
X001XB-I14936-97-1
P012PR32638-88-3
B0265-Br-PAPS81608-06-2(free acid)
N031Nitro-PAPS143205-66-7(as anhydride)

Substrate

Code#DescriptionCAS#
G213Glupa-C63699-78-5
L005L-Leucyl-p-nitroanilide HCl16010-98-3
N055Disodium p-Nitrophenylphosphate333338-18-4(hexahydrate)
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