訊息公告專題產品

專題產品

Dojindo Cell Function 系列產品

Reagents for Improved Analysis of Cellular Functi


Live and Dead Cells

品項規格貨號
細胞增殖/毒性 Cell Proliferation / Cytotoxicity Assay
Cell Counting Kit-8
100-10,000 testsCK04
細胞增殖/毒性 Cell Proliferation / Cytotoxicity Assay
Cell Counting Kit-F
500 tests 
CK06
細胞毒性 Cell Cytotoxicity Assay
Cytotoxicity LDH Assay Kit-WST
500 tests 2,000 tests 
CK12
細胞數校正 Enhance Microplate Assay Data Reliability
Cell Count Normalization Kit
200 tests 1000 tests C544

Cellular Membrane

品項規格貨號
內吞作用 Endocytosis Detection
ECGreen-Endocytosis Detection
40 ulE296
細胞膜染色 Cell Membrane Staining
PlasMem Bright 
100 ul 100 ul P504 (Green) P505 (Red) 
外泌體膜染色 Exosome Membrane Fluorescent Staining ExoSparkler Exosome Membrane Labeling Kit
5 samples* 5 samples* 5 samples*
EX01 (Green) EX02 (Red)    EX03 (Deep Red)
外泌體蛋白染色  Exosome Protein Fluorescent Staining ExoSparkler Exosome Protein Labeling Kit5 samples* 5 samples* 5 samples*
EX04 (Green)
EX05 (Red)
EX06 (Deep Red)

* 1-10 ug protein/sample or 10-100x108 particle/ sample


Autophagy

品項規格貨號
細胞自嗜 Detection of Autophagosome
DAPGreen -Autophagy Detection
5 nmolD676
細胞自嗜 Detection of Autophagosome
DAPRed -Autophagy Detection
5 nmolD677
細胞自嗜 Detection of Autolysosome
DALGreen -Autophagy Detection
20 nmolD675

ROS and Ferroptosis


Clear observation with highly sensitive dye

Detaction conditions

  • Fluorescent microscope : Ex: 450-490 nm, Em: 500-550 nm(GFP filter). Exposure time : DCFH-DA : 1.2s, Highly Sensitivw DCFH-DA : 0.13s
  • Flow cytometer : FITC laser gain: 215 V
  • Microplatereader : Ex. 490 – 520 nm / Em. 510 – 540 nm

Live cell image of lipid peroxide

Lipid peroxide of living cells
Cell line: L929
Microscope: Zeiss LSM510META
Filter type: FITC(GFP, Alexa488)wide filter
HFT UV/488
NFT490
BP505-550


Live cell image of intracellular iron


GSSG/GSH Assay Procedure

品項規格貨號
Total ROS Detection ROS Assay Kit-Highly Sensitive DCFH-DA-100 testsR252
Lipid Peroxide Detection Liperfluo
1set (50 μg x5)L248
Intracellular Iron Dection FerroOrange
24 ug x 1 24 ug x 3F374
Cystine Uptake Assay Kit
100 testsUP05
Reduced (GSH) and Oxidized (GSSG) Glutathione Quantification GSSG/GSH Quantification Kit
200 testsG257

Mitochondria

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品項規格貨號
粒腺體標定 Mitochondria Staining MitoBright LT 系列400 ul x1 400 ul x3MT10 Green MT11  Red MT12 Deep Red
粒腺體膜電位 MMP(Mitochondria Membrane Potential) JC-1 MitoMP Detection kit
1set (96 well x 5, 3.5cm dish x 25) MT09
可固定粒腺體膜電位 MMP(Mitochondria Membrane Potential)
MT-1 MitoMP Detection kit
1set
(3.5cm dish x 30) 
MT13
粒腺體二價鐵Fe2+ (Mitochondria Iron) Mito-FerroGreen
50 ug x 2  M489
粒腺體脂質過氧化 (Lipophilic Peroxide) MitoPeDPP
5 ug x 3  (96 well x 1-5, 3.5cm dish x 5-25) M466
粒腺體單重態氧 (Singlet Oxygen) Si-DMA for Mitochondrial Singlet Oxygen Imaging
2 ug  (8 well slide x 22-90)MT05
粒腺體自嗜 (Mitophagy ) Mitophagy Detection Kit
1 set   (8 well slide x 30)MD01
粒腺體自嗜 (Mitophagy ) Mitophagy Dye
5 ug x 3
MD02

Cellular Metabolism

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品項規格貨號
Glucose Metabolism Assay Glucose Assay Kit-WST200 testsG264
Lactic Acid Measurement Lactate Assay Kit-WST
200 tests L256
NAD+/NADH Assay NAD/NADH Assay Kit-WST
100 tests N509
NADP+/NADPH Assay NADP/NADPH Assay Kit-WST
100 testsN510
Glutamine Assay Glutamine Assay Kit-WST
100 testsG268
Glutamate Assay Glutamate Assay Kit-WST
100 tests G269
a-Ketoglutaric Acid Measurement a-Ketoglutarate Assay Kit-Fluorometric
100 testsK261
ATP Measurement ATP Assay Kit-Luminescence
100 tests A550

Related Products

品項規格貨號
Glucose Uptake Assay Glucose Uptake Assay Kit 1 setUP01  Blue UP02 Green UP03 Red
Amino Acid Uptake Assay Amino Acid Uptake Assay Kit
100 tests UP04
細胞數校正 Enhance Microplate Assay Data Reliability
Cell Count Normalization Kit
200 tests
1000 tests 
C544

Cellular Senescence

X-Gal Method

   Young Cells                 Senescent Cells

Dojindo's SPiDER-βGal

    Young Cells               Senescent Cells


Visualize DNA damage

Green :
λex : 494 nm、λem : 518 nm
Red :
λex : 550 nm、λem : 566 nm
Deep Red :
λex : 646 nm、λem : 668 nm


Visualize nucleolus enlargement

品項規格貨號
β-galactosidase 螢光染劑  SPiDER-βGal20 μg x 3SG02
SA-β-galactosidase 染色kit Cellular Senescence Detection Kit – SPiDER-ßGal10 assaysSG03
SA-β-galactosidase 試驗組 Cellular Senescence Plate Assay Kit - SPiDER-βGal
100 tests SG05
細胞DNA損傷檢測組 DNA Damage Detection Kit - γH2AX系列  1 set  (當染色量為 100 µL, 約可做50次)G265  Green G266  Red G267  Deep Red
細胞核仁螢光染劑 Nucleolus Bright 系列
60 nmole  (3.5cm dish x 30) N511   Green N512   Red
細胞數校正 Enhance Microplate Assay Data Reliability
Cell Count Normalization Kit
200 tests
1000 tests 
C544

Lipid Drop

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品項規格貨號
Lipid Droplet Staining Lipi series  (Blue, Green and Red 8 well slide x 6-62) (Deep Red 8 well slide x 12-62) 10 nmol 10 nmol 100 nmol 10 nmolLD01  Blue  LD02 Green  LD03 Red LD04 Deep-Red 
Lipid Droplet Quantification Lipid Droplet Assay
1 set 96 well plate x1 40 assays for Flow  LD05 Blue LD06 Deep-Red  

Cell Cycle

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品項規格貨號
Quick Detection of the Cell Cycle Cell Cycle Assay Solution Blue50 testsC549
Quick Detection of the Cell Cycle
Cell Cycle Assay Solution Deep Red
50 tests C548